tissue culture Archives - Center for Plant Conservation

Save Plants: March 2024 Newsletter

Posted on March 21, 2024 (March 21, 2024) by Shannon Fowler

Quercus dumosa in flower. Photo by Caroline Iacuaniello

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The Huntington Cryobiotechnology Program: Magnolia Case

Posted on March 21, 2024 (March 21, 2024) by Shannon Fowler

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Conservation Champion: Dr. Joe Ree

Posted on March 20, 2024 (April 9, 2024) by Shannon Fowler

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Asimina manasota in vitro

Asimina manasota plants growing in vitro at the Lindner Center for Conservation and Research of Endangered Wildlife at the Cincinnati Zoo and Botanical Garden (CREW). […]

Propagation of the endangered Del Mar manzanita (Arctostaphylos glandulosa ssp. crassifolia) through tissue culture

The Del Mar manzanita (Arctostaphylos glandulosa ssp. crassifolia) is an endangered perennial shrub native to San Diego County, CA, USA. Although their seeds may be banked for years, germination rates are exceedingly low. Scarification, acid exposure, and heat shock require considerable time and effort to achieve improved germination rates. Tissue culture is a viable alternative. […]

Successful In Vitro Initiation: Taxus floridana

showing new axillary buds in a test tube on June 12, 46 days after inoculation. […]

Shoot Tips for Long-term Preservation

When genebanks must cryopreserve a diverse range of clonally propagated plants, it is often most effective to preserve their shoot tips–tiny growing points in their tissues. USDA’s Remi Bonnart discusses the circumstances for which shoot tip cryopreservation is appropriate, and shows how a variety of shoot tips preservation methods are used to preserve grape, strawberry, […]

An Introduction to Plant Clonal Cryopreservation

Cryopreservation is a technology that allows genebanks to preserve plant genetic resources on a long-term basis. USDA’s Jennifer Kendall discusses how cryopreservation is used at the National Laboratory for Genetic Resources Preservation to preserve clonal material–that is tissue that contains the exact same genetic makeup as the source plant–in liquid nitrogen. This video was made […]

Orchid Micropropagation: What it is, How to Do it, Methods & Challenges

Screenshot of Atlanta Botanic Garden's video on orchid propagation

Orchids are known to be quite difficult to propagate as they typically require fungal symbionts during their germination. In this video, Jason Ligon and Tito Tomei explain how to propagate orchids in the lab using sterile micropropagation techniques. They show each step and emphasize how important sterility is for the process. This video was made […]

Quercus dumosa, Nuttall’s Scrub Oak, Coastal Sage Scrub Oak

San Diego Zoo Wildlife Alliance postdoc, Dr. Joe Ree, presented some of his work with Quercus dumosa at the 2021 Channel Islands and California Native Oak Workshop. Dr. Ree is working with tissue cultures to try and establish a method of propagating this rare scrub oak. He hopes to further preserve the tissues using cryopreservation […]

Conservation Through Cultivation of Live-forevers

Kevin Alison from the Tree of Life Nursery presents Dudleya propagation research that the nursery is currently conducting. He addresses threats the genus Dudleya faces including habitat loss and extreme poaching. Utilizing tissue culture methods, Kevin seeks to bring more of these charismatic species to the nursery trade. He hopes that readily available plants can […]

Strategies to Cryopreserve Embryonic Axes of North American Oaks: Interactions of Moisture, Cryoprotectants and LN Exposure Rates in Quercus imbricaria

Screenshot of Strategies to Cryopreserve Embryonic Axes of North American Oaks: Interactions of Moisture, Cryoprotectants and LN Exposure Rates in Quercus imbricaria video

Jose Faria, Lisa Hill, Christina Walters, Tree Seed Laboratory, Federal University of Lavras, Brazil, USDA-ARS, National Center for Genetic Resources Preservation, United States Quercus imbricaria is included in the red oak group (Lobatae) and is broadly distributed in the Midwestern US. The embryonic axes are about 1 mg dry mass and have 0.68 g H2O/g […]

Ex-Situ Conservation of Endangered Succulent Plants Using Cryobiotechnology Tools: The Aloe and Agave Cases

Screenshot of Ex-Situ Conservation of Endangered Succulent Plants Using Cryobiotechnology Tools video

Josette Tin, Taylor La Val, Sean Lahmeyer, John Trager, Raquel Folgado*, The Huntington Library, Art Collections and Botanical Gardens, San Marino, CA., United States, *Speaker Succulent plants are significant to the horticultural industry, and they are also sources for food, fibers, medicines, and cosmetics. The main threats for the wild population of these often emblematic […]

Assessment of Genetic Stability During Cryopreservation of Vanilla (V. planifolia) Shoot-tips Using ISSR Markers

Maria Teresa Gonzalez-Arnao, Pedro Isidro-Adolfo, Maria F. NetzahualcoyotlMata, Lourdes G. Iglesias Andreu, Jaime Martínez-Castillo, University of Veracruz, INBIOTECA, CICY, In this study we used six selected ISSR primers (T05, T06, C07, UBC823, UBC825, UBC848) to evaluate the genetic stability during cryopreservation of vanilla (V. planifolia) shoot-tips subjected to a Droplet-Vitrification (D-V) protocol using PVS2 solution for dehydration. We […]

Cryopreserving Clonal Crops: Increasing Processing Efficiency in Genebanks

Gayle Volk, USDA ARS National Laboratory for Genetic Resources Preservation, United States The USDA-ARS National Plant Germplasm System has over 30,000 clonally maintained accessions within its field, screenhouse, greenhouse, and tissue culture collections. These fruit, nut, tuber, and bulb crop collections are usually not duplicated at secondary locations and are vulnerable to biotic, abiotic, and climatic threats. Only about 15% of […]

Conservation Champion: Jason Ligon

Posted on June 17, 2019 (December 1, 2020) by Caroline Iacuaniello

Photo of Jason Ligon, Tissue Culture & Seed Bank Coordinator, Atlanta Botanical Garden

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Cryobiotechnology for Maintaining Genetic Diversity of Exceptional Species: The Case of Crotalaria avonensis

Valerie Pence and Megan Philpott, Cincinnati Zoo & Botanical Garden Crotalaria avonensis is a Florida endemic found in three populations and characterized by low seed production. In the late 1990s, CREW developed protocols for tissue culture propagation from field collected shoot cuttings as well as cryopreservation methods. In order to develop a genetically representative collection […]

Conservation Champion: Valerie Pence, Ph.D.,

Posted on March 13, 2019 (December 2, 2020) by Caroline Iacuaniello

photo of Valerie Pence, Ph.D., Director of Plant Research, Center for Conservation and Research of Endangered Wildlife, Cincinnati Zoo and Botanical Garden

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