Quercus dumosa, Nuttall’s Scrub Oak, Coastal Sage Scrub Oak

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San Diego Zoo Wildlife Alliance postdoc, Dr. Joe Ree, presented some of his work with Quercus dumosa at the 2021 Channel Islands and California Native Oak Workshop. Dr. Ree is working with tissue cultures to try and establish a method of propagating this rare scrub oak. He hopes to further preserve the tissues using cryopreservation […]

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Dr. Barbara Reed Interview: Developing Cryopreservation Protocols for Plants

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Dr. Barbara Reed of the US Department of Agriculture, National Clonal Germplasm Repository, has spent her career working with tissue culture and cryopreservation of fruit crops. She was editor of a book, often referred to as ‘the Bible of Cryopreservation’, titled ‘Plant Cryopreservation: a Practical Guide‘. In this video, Dr. Joyce Maschinski questions her about […]

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Dr. Bart Panis Interview: Developing Cryopreservation Protocols for Plants

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Dr. Bart Panis is a renowned plant cryobiologist at Biodiversity International who developed a method of droplet vitrification. This protocol made it possible to cryopreserve the tropical banana, an incredibly difficult species to conserve in collections. In this interview, Dr. Joyce Maschinksi asks him about his methodology, how it can be used to cryopreserve other […]

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Dr. Hugh Pritchard: The Importance of Cryobiology for Plants

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Dr. Hugh Pritchard is a renowned botanist at The Royal Botanic Gardens at Kew. In this interview, conducted by Dr. Joyce Maschinski, he discusses how cryopreservation techniques are necessary to the conservation of important plant species where seed is unable to be stored using traditional seed banking methods. […]

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What makes a good cryobiologist?

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Talented cryobiologists Bart Panis, Shin-Ichi Yamamoto, Meera Das, Daniel Ballesteros, Chris O’ Brian, and Rachel Folgado describe what they believe makes for a good cryobiologist. Among the many qualities they discuss are such attributes as patience, fine motor skills, dedication, enthusiasm, a desire to preserve material for future generations, and above all, passion. […]

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Ex situ Conservation and Ontogenesis of Torreya taxifolia Embryos

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Neusa Steiner, Federal University of Santa Catarina, Emily Coffey, Atlanta Botanical Garden, Jason Ligon, Atlanta Botanical Garden, Lisa Hill, USDA-ARS, Emma Dorr, USDA-ARS, Laurie Blackmore, Atlanta Botanical Garden, Christina Walters, USDA-ARS Torreya taxifolia Arn (Taxaceae) is an ancestral evergreen tree on the brink of extinction. This dioecious plant is found in the Florida panhandle and is threatened by a fungal pathogen. […]

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The Tip of the Iceberg: What We Know and Don’t Know About Exceptional Plants

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Valerie Pence, Cincinnati Zoo & Botanical Garden Plant species that, either through seed availability or physiology, are unable to be conserved and recovered using conventional seed banking practice have been designated as exceptional plants. A definition of exceptionality has recently been put forward, describing four factors that contribute to this condition, depending on what step in […]

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Why is Cryopreservation Important to Endangered Native Plants?

Cryopreservation, a method of storing tissues in liquid nitrogen, is becoming increasingly important to rare plant conservation. This is because many plant species, including large groups such as oaks, orchids, and bryophytes, are unable to be stored long term using more traditional seed banking methods. Using cryopreservation techniques, researchers are able to preserve greater crop […]

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Why is Cryopreservation So Interesting?

Plant Conservationists have found that many plants are unable to be stored using traditional seed banking techniques. To address this issue, researchers are exploring the use of cryopreservation, the storing of tissues in liquid nitrogen, to preserve exceptional species. In this video scientists Daniela Impe, Christina Walters, and Karin Van Der Walt explain how they […]

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The Potential of Shoot Tip Cryopreservation for the Ex Situ Conservation of Oaks.

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Dr. Valerie Pence, Cincinnati Zoo & Botanical Garden Although there are published protocols for initiating shoot cultures for over 20 oak species, the cryopreservation of oak shoot tips has not been reported. We have applied the droplet vitrification protocol to four species of oaks, including the endangered Q. hinckleyi, in order to evaluate the feasibility of […]

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What do bananas, avocados and vanilla have in common?

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Joyce Maschinski, Center for Plant Conservation Joyce Maschinski interviews world famous plant cryo-biologists to learn about how cryopreservation is imperative for the future of food. Many food crops can’t be preserved using traditional seed preservation methods. For some of these species cryopreservation is the only long term option. Plant researchers from across the globe are working […]

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Probing Life and Death in the Glassy State of Cryopreserved Seeds, Fern Spores and Pollen: Fundamental Research to Optimize Ex Situ Preservation

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Dani Ballesteros, Christina Walters, Hugh W. Pritchard Royal Botanic Gardens Kew, Wellcome Trust Millennium Building, United Kingdom, United States Department of Agriculture., United States Often various plant propagules (e.g., seeds, pollen and fern spores) can be stored dry (or partially dry) at sub-zero temperatures for extended periods of time. Under such conditions the cell cytoplasm […]

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Cryopreservation: An Approach for Long-term Storage of Phyto-diversity Plants of North-East India

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Meera Das*, Reema Diengdoh, Seram Devika, Suman Kumaria, North-Eastern Hill University, Shillong, India, *Speaker North-east India, being one of the global hot-spots, is considered the richest reservoir of diverse plant genetic resources. However, the vast utilities of plant species in medicines, ornamentals, fragrances, urbanization and illegitimate harvest of plant genetic resources have immense impact on […]

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Cryo Biotechnology for Somatic Embryos of Agave Tequilana Cv. Chato: An Endangered Genetic Resource

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Lourdes Delgado-Aceves, Maria T. Gonzalez- Arnao, Liberato Portillo, Raquel Folgado, CUCBA-University of Guadalajara, University of Veracruz, The Huntington Library, Art Collections and Botanical Gardens Agave tequilana cv. chato is an important resource widely used to produce a very emblematic and popular Mexican beverage called mezcal; however, its wild populations are currently severely degraded due to […]

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Strategies to Cryopreserve Embryonic Axes of North American Oaks: Interactions of Moisture, Cryoprotectants and LN Exposure Rates in Quercus imbricaria

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Jose Faria, Lisa Hill, Christina Walters, Tree Seed Laboratory, Federal University of Lavras, Brazil, USDA-ARS, National Center for Genetic Resources Preservation, United States Quercus imbricaria is included in the red oak group (Lobatae) and is broadly distributed in the Midwestern US. The embryonic axes are about 1 mg dry mass and have 0.68 g H2O/g […]

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Ex-Situ Conservation of Endangered Succulent Plants Using Cryobiotechnology Tools: The Aloe and Agave Cases

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Josette Tin, Taylor La Val, Sean Lahmeyer, John Trager, Raquel Folgado*, The Huntington Library, Art Collections and Botanical Gardens, San Marino, CA., United States, *Speaker Succulent plants are significant to the horticultural industry, and they are also sources for food, fibers, medicines, and cosmetics. The main threats for the wild population of these often emblematic […]

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