Strategies to Cryopreserve Embryonic Axes of North American Oaks: Interactions of Moisture, Cryoprotectants and LN Exposure Rates in Quercus imbricaria

Screenshot of Strategies to Cryopreserve Embryonic Axes of North American Oaks: Interactions of Moisture, Cryoprotectants and LN Exposure Rates in Quercus imbricaria video

Jose Faria, Lisa Hill, Christina Walters, Tree Seed Laboratory, Federal University of Lavras, Brazil, USDA-ARS, National Center for Genetic Resources Preservation, United States Quercus imbricaria is included in the red oak group (Lobatae) and is broadly distributed in the Midwestern US. The embryonic axes are about 1 mg dry mass and have 0.68 g H2O/g […]

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Ex-Situ Conservation of Endangered Succulent Plants Using Cryobiotechnology Tools: The Aloe and Agave Cases

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Josette Tin, Taylor La Val, Sean Lahmeyer, John Trager, Raquel Folgado*, The Huntington Library, Art Collections and Botanical Gardens, San Marino, CA., United States, *Speaker Succulent plants are significant to the horticultural industry, and they are also sources for food, fibers, medicines, and cosmetics. The main threats for the wild population of these often emblematic […]

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Assessment of Genetic Stability During Cryopreservation of Vanilla (V. planifolia) Shoot-tips Using ISSR Markers

Screenshot of Assessment of Genetic Stability During Cryopreservation of Vanilla (V. planifolia) Shoot-tips Using ISSR Markers video

Maria Teresa Gonzalez-Arnao, Pedro Isidro-Adolfo, Maria F. NetzahualcoyotlMata, Lourdes G. Iglesias Andreu, Jaime Martínez-Castillo, University of Veracruz, INBIOTECA, CICY,  In this study we used six selected ISSR primers (T05, T06, C07, UBC823, UBC825, UBC848) to evaluate the genetic stability during cryopreservation of vanilla (V. planifolia) shoot-tips subjected to a Droplet-Vitrification (D-V) protocol using PVS2 solution for dehydration. We […]

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Cryopreserving Clonal Crops: Increasing Processing Efficiency in Genebanks

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Gayle Volk, USDA ARS National Laboratory for Genetic Resources Preservation, United States The USDA-ARS National Plant Germplasm System has over 30,000 clonally maintained accessions within its field, screenhouse, greenhouse, and tissue culture collections. These fruit, nut, tuber, and bulb crop collections are usually not duplicated at secondary locations and are vulnerable to biotic, abiotic, and climatic threats. Only about 15% of […]

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Cryobiotechnology for maintaining genetic diversity of exceptional species: The case of Crotalaria avonensis

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Valerie Pence and Megan Philpott, Cincinnati Zoo & Botanical Garden Crotalaria avonensis is a Florida endemic found in three populations and characterized by low seed production. In the late 1990s, CREW developed protocols for tissue culture propagation from field collected shoot cuttings as well as cryopreservation methods. In order to develop a genetically representative collection […]

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Conserving Wild Exceptional Species Case Study: Crotalaria avonensis

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Joyce Maschinski, Center for Plant Conservation and Valerie Pence, Cincinnati Zoo & Botanical Garden Crotalaria avonensis is an endangered legume endemic to Florida that produces few seeds. In vitro shoot cultures of multiple genotypes have been grown at CREW to provide genetic diversity for restoration and for tissue cryopreservation. These cultures harbor a bacterium, identified […]

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Cryopreserving Endangered Plants and Seeds

Although many rare plants have seeds that can be stored by conventional methods, not all species have seeds that can live after drying or freezing. Sometimes called “Exceptional plants, ” these species have a wide range of variation. Some produce few or no seeds, thus they cannot be seed banked, others have seeds or spores […]

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In Vitro Seed Germination and Seedling Development of Spiranthes Casei for Ex Situ Conservation

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Peter Zale and Matt Taylor, Longwood Gardens Several species of Spiranthes native to the Eastern U.S. are considered rare, threatened or endangered by federal and state agencies. Using the Pennsylvania endangered Spiranthes casei as a model species, experiments were designed to determine optimal conditions for in vitro seed germination and seedling development. Seeds were collected […]

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