How to Prepare a Saturated Salt Solution to Dry Seeds

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Lisa Hill – National Laboratory for Genetic Resources Preservation (USDA-ARS) Drying seeds to appropriate relative humidity is the first step to long term seed storage. NLGRP and CPC recommend drying seeds to 25-35% relative humidity at room temperature. One way to acheive specific relative humidities is to use saturated salt solutions. Here, Lisa shows the […]

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Revealing the Details of Hawaiian Seeds Through Microscope Photography

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Tim Kroessig, Harold L. Lyon Arboretum The Hawaiian flora represents ~45% of all plants listed by the U.S. Fish and Wildlife Service as threatened or endangered. The Lyon Arboretum’s Seed Conservation Laboratory (Lyon S.C.L.) conserves many of these imperiled plants through conventional seed banking. However, the seeds of many rare Hawaiian plants have never been […]

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It’s Complicated: The Launch of a New Seed Banking Project

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Jim Locklear, Lauritzen Gardens Blowout penstemon (Penstemon haydenii) is a critically imperiled (G1) plant listed as an endangered species by the USFWS. It is presently known from ten occurrences in the Nebraska Sandhills and three in the Ferris Dunes of Wyoming. This species is associated with areas of active wind erosion within a grassland matrix. […]

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How to Test Seeds for Germination

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Germination Testing Program Cheryl Birker, Rancho Santa Ana Botanic Garden The California Seed Bank at Rancho Santa Ana Botanic Garden has a germination testing program to monitor the viability of its many conservation seed collections. Germination tests are conducted on all incoming seed collections before they are placed in freezer storage, and for all rare […]

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Special considerations in the seed collections of two NC rare plant species

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Recently updated Best Plant Conservation Practices to Support Species Survival in the Wild detail guidelines for seed collection of rare plants. These include multiple aspects to consider in making seed collections regarding target species characteristics, collection timing, seed collection amount, population genetics and maintaining site quality. Here we outline seed collections for two species for […]

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Conserving the Alpine: From Tiny Seeds to Big Collaboration

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Jennifer Ramp Neale, Denver Botanic Gardens Alpine plants are at risk of population decline and/or extinction due to climate change. Understanding these plants and the environments in which they survive and thrive involves a multi-tiered approach including in-situ and ex-situ efforts. At Denver Botanic Gardens we are working to collect and study seed of alpine […]

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All in the family – the case for collecting by maternal lines

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Conservation seed collections support species’ survival by acting as an insurance policy in the face of extinction. They can also provide resources for research, restoration and reintroduction. A high-quality conservation seed collection has both depth and breadth – capturing genetic diversity within and geographic diversity among populations. Collecting and storing seeds by maternal line (i.e., […]

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Range-wide Ex Situ Seed Conservation and Population Genetic Architecture Analysis in Venus Flytrap

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Johnny Randall and Michael Kunz (North Carolina Botanical Garden, University of North Carolina at Chapel Hill), and Jamie Winshell, Corbin D. Jones and Gregory P. Copenhaver (Department of Biology and Integrative Program for Biological & Genome Sciences,University of North Carolina at Chapel Hill) Venus’ flytrap (Dionaea muscipula) is the most widely recognized carnivorous plant, and […]

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NAOCC’s Collaborative Orchid Seed Storage Project

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Dennis Whigham and Julianne McGuinness, North American Orchid Conservation Center The North American Orchid Conservation Center (NAOCC) was developed by the Smithsonian and the U.S. Botanic Garden to conserve the diversity of native orchids in the U.S. and Canada. NAOCC ecologically-based conservation model has three guiding principles: Preservation through seed and fungal banks, Propagation, Education. NAOCC […]

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Lessons Learned from the Kew MSB Seed Conservation Techniques Training Course

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Heather Schneider, Santa Barbara Botanic Garden In the fall of 2017, Dr. Heather Schneider from the Santa Barbara Botanic Garden attended the Kew Millennium Seed Bank Partnership’s three-week Seed Conservation Techniques Training Course. The course brought together conservationists from all over the world to improve conservation seed banking practices used by MSB partners. The course […]

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Penstemon peckii Soil Seed Bank Study at 25 Years

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Ed Guerrant, Rae Selling Berry Seed Bank Penstemon peckii (G3-S3 Federal SOC) is an Oregon endemic with a relatively compact range in the semi-arid Ponderosa pine forest east of the Cascade Mountains. The vast majority of known populations (ca 93%) are almost entirely within the Sisters Ranger District of the Deschutes National Forest. A 1992 […]

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Why Seeds and Spores Die in the Freezer and Sometimes Faster That Anticipated

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Chris Walters, Research Leader of the Plant Germplasm Preservation Research team at USDA-ARS National Laboratory for Genetic Resources Preservation Knowing how long storedgermplasmsurvives is critical for effective banking of genetic resources. Longevity is inherently difficult to predict because there are so many factors controlling how cells respond to storage conditions. Uncertainty increases forgermplasmcollections of natural […]

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Seed conservation of Abies bracteata at Rancho Santa Ana Botanic Garden

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Cheryl Birker, Seed Conservation Program Manager and Evan Meyer, Rancho Santa Ana Botanic Garden Abies bracteata(Bristlecone fir; Santa Lucia fir) is a 12-30 meter tall tree restricted to a small, wildfire prone range in the Santa Lucia Mountains on the central coast of California. While several botanical gardens maintain living specimens, it remains rare in […]

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