Cryopreserving Clonal Crops: Increasing Processing Efficiency in Genebanks

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Gayle Volk, USDA ARS National Laboratory for Genetic Resources Preservation, United States The USDA-ARS National Plant Germplasm System has over 30,000 clonally maintained accessions within its field, screenhouse, greenhouse, and tissue culture collections. These fruit, nut, tuber, and bulb crop collections are usually not duplicated at secondary locations and are vulnerable to biotic, abiotic, and climatic threats. Only about 15% of […]

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The Frozen Zoo® of San Diego Zoo Global: Past, Present, and Future

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Dr. Oliver Ryder, Barbara Durrant, Marlys L. Houck, Marisa Korody, Cynthia Steiner, Institute for Conservation Research, San Diego Zoo Global Cryobanking of viable tissue culture cells at the San Diego Zoo, named the “Frozen Zoo® by its founder, Kurt Benirschke, has contributed knowledge advancing conservation science and species recovery from its beginnings in 1975. This […]

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Why Seeds and Spores Die in the Freezer and Sometimes Faster That Anticipated

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Chris Walters, Research Leader of the Plant Germplasm Preservation Research team at USDA-ARS National Laboratory for Genetic Resources Preservation Knowing how long storedgermplasmsurvives is critical for effective banking of genetic resources. Longevity is inherently difficult to predict because there are so many factors controlling how cells respond to storage conditions. Uncertainty increases forgermplasmcollections of natural […]

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