Seed germination trials were done at the Berry Botanic Garden. Three batches of seeds (11, 12, and 16 years old) were subjected to 4 sets of environmental conditions with two variables: length of cold stratification and growth chamber temperature regime. Cold stratification for 8 or 16 weeks followed by 68F (20C) or 50/68F (10/20C) treatment resulted in 20-100% germination depending on the parent plant. No one treatment was consistently better than the others. The 16 year old accession, stored since the first year the seed bank was in operation (1983), yielded a maximum of 90% germination (8 weeks of cold stratification followed by alternating 10/20C (50/68F) (Gasser 1999).

Seeds from 3 locations in Jackson County (Agate Desert) and 2 locations in Josephine County (French Flat) are stored at The Berry Botanic Garden.

Seeds from 3 locations in Jackson County (Agate Desert) and 2 locations in Josephine County (French Flat) are stored at The Berry Botanic Garden.

A narrow endemic, restricted to 2 small valley bottoms in Jackson and Josephine counties in southwestern Oregon. Fairly complete surveys of this species' limited vernal pond habitat have been conducted and 32 occurrences were found with a total of about 50,000 reproductively mature plants. Both of the areas in which the species occurs are being threatened by residential and urban development and one area is also threatened by mining and off-road vehicle traffic.

Habitat loss due to industrial, commercial, agricultural, and residential development (USFWS 2000a). Changes in hydrology of vernal pools due to: ORV use. When driven in moist areas, ORV tires create large ruts and can fracture the clay hard pan la

As of 2000: 13 occurrences in the Agate Desert, Jackson County and 10 in French Flat, Josephine County. Total occupied habitat is about 200 acres. Mapped potential habitat totals 133 acres in the Agate Desert, but recent disturbances have limited occupied habitat to approximately 69 acres. In French Flat, about 150 acres of habitat is occupied (USFWS 2000a). The largest French Flat population consists of an estimated 207,000 individuals, while many populations contain fewer than 50 (Kaye 2000a, 2000b).

Seed germination trials were done at the Berry Botanic Garden. Three batches of seeds (11, 12, and 16 years old) were subjected to 4 sets of environmental conditions with two variables: length of cold stratification and growth chamber temperature regime. Cold stratification for 8 or 16 weeks followed by 68F (20C) or 50/68F (10/20C) treatment resulted in 20-100% germination depending on the parent plant. No one treatment was consistently better than the others. The 16 year old accession, stored since the first year the seed bank was in operation (1983), yielded a maximum of 90% germination (8 weeks of cold stratification followed by alternating 10/20C (50/68F) (Gasser 1999). Demographic monitoring from 1994 through 1999 at populations on Bureau of Land Management (BLM) land. Transition matrices were prepared to assist in determining population trends (Kaye 2000a, 2000b). Preliminary genetic work (using AFLPs) has not revealed any differences between the Agate Desert and French Flat populations (Gitzendanner, M. at Washington State University (now at the University of Florida)). A study of effective management tools. Plots containing Lomatium. cookii were subjected to one of three treatments: raked, raked and scarified, or left as a control. Germination and seedling survivorship were greatest in the raked plots (D. Borgias in U.S. Fish and Wildlife Service (USFWS) 2000a).

Three populations occur on Oregon Department of Transportation (ODOT) Land. According to the ODOT website, they are under ""special management"" The largest known population is in the Agate Desert and is managed by The Nature Conservancy. Here, they manage approximately 17 acres of Lomatium cookii habitat. The Nature Conservancy attempted to salvage 140 plants from an area that was to be graded for road construction on private property. Although the landowner approved removal of the plants, only one individual was salvaged before grading occurred. It died after transplantation (USFWS 2000a). Seeds from 3 locations in Jackson County (Agate Desert) and 2 locations in Josephine County (French Flat) are stored at The Berry Botanic Garden. One site in French Flat is designated as an Area of Critical Environmental Concern (ACEC).

Fence land to restrict access. Restrict Off-Road Vehicle (ORV) use on BLM land and Agate Desert Preserve. Mowing, burning, light grazing or raking of habitat after seed maturation but before fall growth to reduce plant cover from exotic grasses (Brock in USFWS 2000a). Continued monitoring on managed lands (BLM land and Agate Desert Preserve).

Collect and store adequate numbers of genetically representative seeds. Determine germination requirements. Initial germination trials were inconclusive. Attempt with larger sample sizes. Determine propagation and re-introduction protocols.