Free Virtual Symposium: Conserving Exceptional Plants – Cryobiotechnologies, Status, and Scale

[Megan Philpott]

The Cincinnati Zoo & Botanical Garden Center for Conservation & Research of Endangered Wildlife (CREW) is hosting a free virtual symposium and workshop: “Conserving Exceptional Plants – Cryobiotechnologies, Status, and Scale” April 8-9, 2025. Participants will hear from experts and participate in discussions on the current state and future of exceptional plant conservation around the world. Topics covered will include:

Updates on tissue and seed cryopreservation research
Updates on the global state of exceptional plant research
What it will take to scale up research and cryobanking of exceptional species
How to set priorities and fill gaps in the field

Participants are encouraged to register to attend and/or submit a poster abstract here: https://cincinnatizoo.org/exceptional-plant-conservation-network/#ceps. Registration ends April 7, 2025. Abstract submission ends March 15, 2025. For any questions, please contact epcn@cincinnatizoo.org.

[ANA MARTINEZ]

Introduction: This study evaluated the impact of different cytokinin concentrations, such as 6-Benzylaminopurine (BAP) and Thidiazuron (TDZ), on the in vitro development of meristematic apices and nodal segments of Coffea arabica L.; variety IAC 144.

Objective: To assess the in vitro development of meristematic apices and nodal segments of Coffea arabica L.; as well as the effects of BAP and TDZ on seedling growth through non-destructive analyses using the ImageJ® software.

Materials and Methods: Explants were cultured in MS medium supplemented with sucrose (30 g L⁻¹) and agar (7 g L⁻¹); at pH 5.8. The environmental conditions were controlled (25 ± 1 °C; 16-hour photoperiod; and 13 µmol m⁻² s⁻¹ light intensity). The BAP treatments included 0; 1.1; 2.2; 3.3; and 4.4 µM; with the latter also supplemented with 1.4 µM IAA. For TDZ; the concentrations tested were 0; 1.1; 2.2; 3.4; 4.5; and 9.8 µM. Evaluations were conducted at 30 and 60 days; analyzing survival; number of shoots and leaves; as well as shoot and leaf length.

Results: BAP was more effective than TDZ in inducing shoot formation and promoting explant development. The concentration of 3.33 µM BAP resulted in the highest number of shoots and leaves. TDZ showed promising results at higher concentrations (4.50 µM and 9.8 µM); inducing shoots in nodal segments. After 60 days; explants treated with BAP exhibited greater shoot growth and leaf production; whereas those treated with TDZ demonstrated potential for complementary propagation protocols.

Conclusion: The use of cytokinins; particularly BAP; proved to be effective in the in vitro propagation of Coffea arabica L.; variety IAC 144; contributing to the optimization of multiplication and conservation techniques for this species. These findings may aid in the refinement of protocols for commercial propagation and germplasm conservation; maximizing in vitro cultivation efficiency and the viability of regenerated plants.

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