FAQ: What do I need to get started with tissue culture?

[Center for Plant Conservation]

[Center for Plant Conservation]

Tissue culture techniques require special expertise, staff time, and additional infrastructure. A sterile location is recommended for ensuring that the plants you are growing in vitro are free of contaminants. A laminar flow hood will help keep contaminants away from the plants as you plant them in the media. You will also need to be able to control temperature and light intensity in your sterile space. Sterile containers are needed (and these can be newly purchased or autoclaved) as well as a variety of other tools, planting media, and hormones. And of course, your organization will need staff trained in using the equipment. Current staff can be trained through a variety of avenues. Working with partners or will allow you to learn about the processes and/or allow you to address intermittent needs without starting your own program. Hiring commercial contractors for in vitro propagation of rare plants presents another option.

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