CENTER FOR PLANT CONSERVATION
February 2018 Newsletter
Thank you as always for opening up another installment of Save Plants. This month, in honor of the recent Valentine’s Day, we recount the enthralling and sometimes tawdry sex life of plants. It is really a sordid affair, involving often beautiful flowers, multiple species, and false promises with little chance of reward. In other words, a normal day in the life of plants. Don’t believe me? Well, read on to learn more about how plants have co-evolved with many different animal species in the ultimate goal of reproducing. Plants and their pollinators are intricately linked and combined, providing untold benefits to humankind. So, here’s to the birds and the bees . . . and flies, beetles, and even mosquitoes that work with our plants to do all that they do for all of us.
Mystery of Flowers Unveiled: Reproduction to Parenting
William (Ned) Friedman, Arnold Professor of Organismic and Evolutionary Biology and Director of The Arnold Arboretum of Harvard University
Flowers play a central role in the reproductive cycle of plants. Their colors, shape, fragrance, and nectar are all designed to attract insects and other pollinators to carry the genetic material from flower to flower. While flowering plants are the largest group of land plants and the type of plants most familiar to us, they still hold much mystery as to how they evolved. Recent research findings shed light on some of these ancient flowering plants.
For 30 years, William (Ned) Friedman has been investigating a longstanding set of questions about the origin and early evolutionary history of flowering plants, Darwin’s so-called “abominable mystery.” His research has involved plant species native to the Namib and Sonoran deserts; rainforests of Queensland, New Caledonia; and most recently equatorial Africa. Many of the plants that form the foundation of this research program have been ex situ specimens in botanical gardens and university greenhouse collections. Indeed, some of the key taxa central to reconstructing the evolutionary history of plants can only be studied in garden and greenhouse collections because they are extinct in the wild.
Research from Friedman’s lab that was announced last week in the Proceedings of the Royal Society B uses just such a species, Nymphaea thermarum, a water lily from equatorial Africa. Sadly, as a consequence of habitat degradation, this beautiful water lily lives on only in botanical gardens or research greenhouses.
Reproductive Biology of Nymphaea thermarum
Flowers of Nymphaea thermarum are hermaphroditic. The flowers open and close over the course of several days. However, unlike most other water lilies where male and female functions occur on different days, in N. thermarum pollen release overlaps with the female phase, marked with the secretion of a prominent drop of fluid that covers the receptive stigmatic surface. Indications are that N. thermarum is predisposed towards self-pollination.
This process is important for the evolution and cultivation of this species:
- N. thermarum can self-fertilize. This may mean that remaining populations have little genetic diversity.
- Flowers can be emasculated by removing the anthers at least 24 hours before the bud first opens.
What happens after reproduction is also important to the story of N. thermarum. Rebecca Povilus (recent doctoral student, Harvard University), Pamela Diggle (professor, University of Connecticut), and Friedman, took advantage of the uniquely brief life cycle of N. thermarum to investigate the theory of inter-parental conflict in plants.
Disagreements between mothers and fathers about how best to rear progeny is not restricted to humans—the battle of the sexes extends to plants too. In their paper “Evidence for parent-of-origin effects and interparental conflict in seeds of an ancient flowering plant lineage,” Povilus, Diggle, and Friedman demonstrate that in a member of one of the oldest flowering plant lineages still in existence, mothers (seed-bearing plants) and fathers (pollen-producing plants) each try to outsmart each other and distribute maternal resources to progeny in order to maximize their own fitness. Fathers display selfish behavior and attempt to drive maternal resources to their own progeny, while mothers take a more balanced view of what is universally best for all of her offspring.
This is the second piece of recent research from the Friedman lab to study an extinct-in-the-wild species. Working with another graduate student, Kristel Schoonderwoerd, the unusual reproductive biology of Franklinia alatamaha (Franklin tree) was analyzed for the first time and published in the Botanical Journal of the Linnean Society. If not for the efforts of William Bartram, who collected Franklinia seed in the late 1700s and introduced this species into gardens in Europe and North America, this extraordinarily beautiful late summer and fall flowering member of the tea family (Theaceae) would be unknown to us, having last been seen in the wild in 1803. All of this is a stark reminder that the conservation efforts of botanical gardens around the world are not only critical to preserving biodiversity, but also to unlocking the secrets of plants and their unique evolutionary history.
Photos of the inside of the seeds (taken with a confocal microscope). They both show the inside of mature or nearly-mature seeds – one shows the whole seed, one is a close-up of part of the seed. For scale, the seeds are about 1-2 millimeters long. The pictures show the major tissue types within a water lily seed: an embryo, it’s sibling tissue (‘endosperm’), a nutrient-storage tissue derived from the mother, and a fuzzy, hair-covered seed coat. (Photos @Rebecca Povilus)
Content provided by William (Ned) Friedman
(Reproductive Biology of Nymphaea thermarum taken from https://nthermarum.weebly.com/reproductive-biology.html, a website created and maintained by Rebecca Povilus)
For further reading:
“Floral biology and ovule and seed ontogeny of Nymphaea thermarum, a water lily at the brink of extinction with potential as a model system for basal angiosperms.” Rebecca A. Povilus Juan M. Losada William E. Friedman. Annals of Botany, Volume 115, Issue 2, 1 February 2015, Pages 211–226.
“Evidence for parent-of-origin effects and interparental conflict in seeds of an ancient flowering plant lineage,” Rebecca A. Povilus, Pamela K. Diggle, William E. Friedman. Proceedings of the Royal Society B, volume 285, issue 1872, 7 February 2018.
Who’s Your Pollinator?
Seana Walsh, National Tropical Botanical Garden
Rappelling down cliffs on Kauai, sometimes suspended a thousand feet above the Pacific Ocean, botanists hand pollinated the critically endangered Hawaiian flowering plant, Brighamia insignis (Alula) in an attempt to save them now that its native pollinator is all but extinct.
There had been speculation that the natural pollinator of both Brighamia species was a native hawk moth that is now either extremely rare or extinct. No hawk moth had ever been observed visiting either species. To determine if the natural pollinator was a hawkmoth, Seana Walsh completed her master’s thesis research on the floral biology, breeding system, pollination ecology, and ex situ genetic diversity of Alula. Building on the four decades of work by botanists Steve Perlman and Ken Wood, Walsh examined floral traits to support or refute the hypothesis that Alula evolved a moth pollination syndrome. She conducted pollen manipulations on cultivated plants to assess the hypothesis that Alula is self-incompatible, that is, it cannot self- pollinate.
Alula is endemic to the island of Kaua‘i and historically to Ni‘ihau. Moth pollination for Alula was proposed based on a few floral trait observations; however, no moth pollinator has ever been observed visiting Alula flowers. There are three native endemic species of moths commonly known as hawk moths, sphinx moths, and hornworms that are reported to occur on Kaua‘i, but other species may have gone extinct. These three species are Hyles calida, Manduca blackburni, and Tinostoma smaragditis. Tinostoma smaragditis (or the Fabulous Green Sphinx of Kaua‘i) is only known from about 19 collections and was last seen in 2000. It is not unreasonable to speculate that the Fabulous Green Sphinx of Kaua‘i could be the single or one of several, native pollinator(s) of Alula.
Examining floral traits provided support for the hypothesis that Alula is adapted to moth pollination. Overall results of pollination treatments suggested a breeding system that was primarily based on cross-fertilization. The results of manipulative pollinations were difficult to interpret with any confidence, however. The quantity and quality of pollen produced was extremely low. Based on 29.5 daytime and 21.5 nighttime floral observation hours, it appears unlikely that anything is effectively pollinating Alula outplantings at the National Tropical Botanical Garden’s Limahuli Garden & Preserve. No moths were observed serving as pollinators. Alula faces severe challenges to reproduce on its own.
(Content provided by Seana Walsh)
Endangered Hawaiian flowering plant, Brighamia insignis (Alula). All of these pictures are of Brighamia insignis (Alula) in cultivation. Images provided by Seana Walsh at the National Tropical Botanical Garden.
Student rappelling down cliffs to reach plants. Images provided by Ken Wood at the National Tropical Botanical Garden.
Cheryl Peterson, Bok Tower Gardens
A thorny, deciduous shrub called Florida Zizphus (Zizphus celata) can only be found in two counties of Lake Wales Ridge in central Florida. The type specimen was collected in 1948, but apparently the species was not seen again and was presumed to be extinct, until Kris Delaney rediscovered a small group of plants in 1987. By 2001, eight other small populations were found.
Although adult plants flower profusely, with tiny yellow-green bisexual flowers that attract numerous diverse insect species, no fruit production occurred in the wild. Genetic research through Archbold Biological Station and the University of Florida showed that these remnant populations are clonal and generally composed of a single genetic individual. They are incapable of producing viable seeds. The good news was that the different populations contained different genotypes. Although research showed many of the genotypes to be cross-incompatible (not able to cross-pollinate), it also showed that there were at least three cross-compatible mating types.
Bok Tower Gardens began propagating clones from the various populations and created a captive population that by the mid-1990s began to produce an annual fruit crop. This allowed the open cross-pollination of compatible genotypes. However, low overall genetic variation remained a problem to species recovery. Then in 2007 five more populations were located, which included what are now two of the largest populations known, and which tripled the number of wild genotypes. The largest population even contained at least new mating types and produced some fruit, although no seedlings have ever been seen in the wild.
The addition of the new genotypes to the captive population at Bok Tower Gardens is underway and will be extremely important to the long-term preservation of the species. Seeds produced from crosses of all genotypes will be the best way to preserve the genetic diversity remaining in the species, both in the ex situ National Collection at Bok Tower Gardens, and in situ through population introductions. Already, seedlings propagated from many years of seed collection from the captive and the one reproductive wild population, representing the most genetic diversity available to date, have been used to establish new populations on protected lands. As these newly-created populations mature and become reproductive, it is hoped they will produce seeds and eventually seedlings, and therefore be self-sustaining to preserve the species.
(Content provided by Cheryl Peterson. Image: Flowers on a Ziziphus celata specimen in the National Collection, January 2018)
Top Left: Pollinator visiting Ziziphus celata in the National Collection in January 2018; Bottom Left: Pollinator on flower of Ziziphus celata in the National Collection at Bok Tower Gardens. ; Right: Flowering Ziziphus celata in the National Collection with Bok Tower in the background
Desert Botanical Garden
George is an endangered Florida semaphore cactus, Consolea corallicola, a prickly pear native to the Florida Keys. But this species can no longer reproduce, leaving George lonely.
Lonely George came to the Desert Botanical Garden as part of a backup collection. The Fairchild Tropical Garden in Florida has primary responsibility for George, but it is a “best practice” to have a backup collection elsewhere.
Why did George lose reproduction ability? There is no clear answer, but there are theories. One of those theories is based on the fact that George is a hexaploid plant with six complete sets of chromosomes. “Sometimes, when there are multiple copies of chromosomes they do not sort properly when the ovules and sperm are formed. Then when fertilization happens, it is impossible for a seed to form.”
Content from website.
Kimberlie McCue, assistant director of research, conservation and collections, states, “By maintaining a healthy collection of Lonely George and telling his story, the garden is able to bring attention to conservation on a larger scale and share common threats that may affect other cactus species.”
The Desert Botanical Garden set up a display as part of the garden’s Cactomania initiative. As guests entered the garden, they were greeted by a multi-sided display featuring Cactomania information and “Meet Lonely George.” The display told the story of the endangered Florida semaphore cactus and encouraged visitors to“Help Find George Friends” by snapping a photograph of their favorite cactus at the garden and sharing on social media with #friendsforgeorge. The garden printed and posted pictures that people had submitted, and created a collage of visitor photographs on the display. The display also included one of the specimens. Check out the Lonely George video here.
Matthew Keir, Botanist, recently part of The Hawaiʻi Conservation Alliance Foundation, started his new position as Division of Forestry and Wildlife (DOFAW) State Botanist and wanted to pass along his contact information. Please contact him if you have any questions or concerns about State T&E permits, commercial plant sales, or any other rare plant work with DOFAW. You can reach Matthew at email@example.com.
February 21, 2018 – Registration Open for Center for Plant Conservation National Meeting. May 3-5, 2018 – Fort Worth, Texas, at the Botanical Research Institute of Texas.
March 6-8, 2018 – Southeast Biodiversity Conservation Forum at the North Carolina Botanical Garden in Chapel Hill, North Carolina. More information here.
April 12-13, 2018 – Florida Rare Plant Task Force Meeting. “The Impact of Climate Change and Natural Disasters on Rare Plant Conservation” a Jacksonville Zoo and Gardens, Jacksonville, Florida. More information here.
May 15-18, 2018 – Hawai’i Native Seed conference and Hawai’i Seed Bank Partnership Meeting, University of Hawai’i at Mānoa.
The Hawai‘i Native Seed Conference brings together conservationists, horticulturalists, researchers, and others working with seeds of native Hawaiian plants to share knowledge with each other and visiting experts. The conference will begin with an optional meeting of the Hawai‘i Seed Bank Partnership on May 15. The following days will feature speakers from Hawaiʻi, and several sessions led by staff from the Kew Millennium Seed Bank Partnership. Topics for the conference will focus on strategies and methods for collecting, handling, and processing seed collections for propagation and storage, making this widely applicable for anyone working in ecosystem restoration in the Pacific region. Abstracts will be accepted through March 11, 2018. Updates available here.
May 18, 2018 – Smithsonian Botanical Symposium, Washington DC, “Plants in the Past: Fossils and the Future.” Presented by the Smithsonian National Museum of Natural History’s Department of Botany in collaboration with the United States Botanic Garden. More information here.
June 4-8, 2018 – American Public Gardens Association Annual Conference, “Cultivate Your Creative Nature” in Southern California. More information here.
July 30-August 1, 2018 – 2nd Seed Longevity Workshop, Fort Collins, CO. Hosted by the International Society for Seed Science (ISSS), USDA Agricultural Research Service (ARS), and Colorado State University (CSU). More information here.
- The Hawai‘i Plant Conservation Network is seeking a new Coordinator. Laukahi is a nonprofit entity that operates under guidance from an elected advisory council and employs a network coordinator to lead the planning, fundraising, project development, coordination, and collaborative work of the Hawaiʻi Plant Conservation Network.
Employer : The Hawaiʻi Conservation Alliance Foundation. Application Instructions : Email cover letter, résumé, and contact information for three references to: firstname.lastname@example.org.
Please see the full job announcement here.
- Smithsonian Gardens is seeking a trained horticulturist with extensive knowledge of orchids to oversee the health, maintenance, and documentation of orchids, and operate active Integrated Pest Management and virus mitigation programs focused on highest level of best practices and standards in plant health care and hygiene. The Orchid Collection Horticulturist will actively participate in educational outreach activities including tours and presentations and oversee the training and management of volunteers and interns. If you are currently a federal employee, veteran, military spouse, or participating in the Peace Corps and AmeriCorps VISTA, click here. General public, click here.
- The Gila Watershed Partnership is looking for an innovative and team-oriented manager for the Gila Native Plant Nursery, a greenhouse and nursery dedicated to providing native plants and seeds for numerous restoration and education projects. The Nursery Manager is responsible for building the capacity of the nursery as well as managing the daily operations. The position requires the management of interns and volunteers, maintenance of facilities, and horticultural responsibilities. The Nursery Manager will ensure the availability of container stock for numerous ecological restoration projects, carry out the nursery business plan, and work to expand the customer base and retail inventory. More information here.
- Research Ecologist GS-0408-12 with the U.S. Geological Survey in Flagstaff, AZ. The position is open to US citizens until March 6, 2018. More information here.
- Assistant Research Professor, Tree Improvement and Genetics. Non-tenure track, 12-month appointment. Salary is commensurate with experience. A PhD is required. At least one degree in Forestry with an emphasis in forest genetics and tree improvement is also required. To apply, submit a letter of application, curriculum vitae, academic transcripts, a description of research interests, and names and contact information for three references to: Michael Gold, School of Natural Resources, 203 ABNR, University of Missouri, Columbia, MO 65211, or email: email@example.com. For more information see: http://www.snr.missouri.edu/ and www.centerforagroforestry.org.
- Conservation Coordinator for the Tennessee Plant Conservation Alliance within the Dept. of Biology and Center for Field Biology, Austin Peay State University. More information here.
The Washington Native Plant Society (WNPS) Conservation Committee is now accepting grant applications for on-the-ground projects that will restore, improve, or support functioning native plant ecosystems in Washington state. Successfully funded projects must provide public benefit and align with conservation principles identified in the WNPS Conservation Statement. Funds will be awarded based on the committee’s determination of which projects would bring the most significant ecological benefit and the likelihood of a project’s long-term success.
Grant applications for 2018 will be accepted through March 1; responses will be sent by April 1. Requests up to $1500 (USD) will be considered, as will smaller requests. Funded projects will be featured in the WNPS flagship journal, Douglasia. Application information here. For questions please contact Becky Chaney, Conservation Committee Chair: 425-880-4220 or firstname.lastname@example.org.
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